Publications

2017

William J. Griffiths, Jonas Abdel-Khalik, Eylan Yutuc, Alwena H. Morgan, Ian Gilmore, Thomas Hearn, Yuqin Wang. Cholesterolomics: An update Analytical Biochemistry 10 January 2017 http://dx.doi.org/10.1016/j.ab.2017.01.009

2016

Peter J. Crick William J. Griffiths, Juan Zhang, Martin Beibel, Jonas Abdel-Khalik, Jens Kuhle, Andreas W. Sailer, Yuqin Wang. Reduced Plasma Levels of 25-Hydroxycholesterol and Increased Cerebrospinal Fluid Levels of Bile Acid Precursors in Multiple Sclerosis Patients Mol Neurobiol 23 November 2016. doi:10.1007/s12035-016-0281-9

Jonas Abdel-Khalik, Eylan Yutuc, Peter J. Crick, Jan-Åke Gustafsson, Margaret Warner, Gustavo Roman, Kevin Talbot, Elizabeth Gray, William J. Griffiths, Martin R. Turner and Yuqin Wang. Defective cholesterol metabolism in amyotrophic lateral sclerosis. The Journal of Lipid Research. November 3, 2016, doi: 10.1194/jlr.P071639

Kurowska-Stolarska M, Hasoo MK, Welsh DJ, Stewart L, McIntyre D, Morton BE, Johnstone S, Miller AM, Asquith DL, Millar NL, Millar AB, Feghali-Bostwick CA, Hirani N, Crick PJ, Wang Y, Griffiths WJ, McInnes IB, McSharry C. The role of microRNA-155/LXR pathway in experimental and Idiopathic Pulmonary Fibrosis. J Allergy Clin Immunol. 2016 Oct 13. pii: S0091-6749(16)31132-0. doi: 10.1016/j.jaci.2016.09.021.

William J. Griffiths, Jonas Abdel-Khalik, Thomas Hearn, Eylan Yutuc, Alwena H. Morgan, Yuqin Wang, Current trends in oxysterol research, Biochemical Society Transactions, 44(2)652-658, April 2016, doi:10.1042/BST20150255

William J. Griffiths, Jonas Abdel-Khalik, Peter J. Crick, Michael Ogundare, Cedric H. Shackleton, Karin Tuschl, Mei Kwun Kwok, Brian W. Bigger, Andrew A. Morris, Akira Honda, Libin Xu, Ned A. Porter, Ingemar Björkhem, Peter T. Clayton, Yuqin Wang, Sterols and oxysterols in plasma from Smith-Lemli-Opitz syndrome patients, The Journal of Steroid Biochemistry and Molecular Biology, March 2016, http://dx.doi.org/10.1016/j.jsbmb.2016.03.018

Kevin A. Robertson , Wei Yuan Hsieh, Thorsten Forster, Mathieu Blanc, Hongjin Lu, Peter J. Crick, Eylan Yutuc, Steven Watterson, Kimberly Martin, Samantha J. Griffiths, Anton J. Enright, Mami Yamamoto, Madapura M. Pradeepa, Kimberly A. Lennox, Mark A. Behlke, Simon Talbot, Jürgen Haas, Lars Dölken, William J. Griffiths, Yuqin Wang, Ana Angulo, Peter Ghazal, An Interferon Regulated MicroRNA Provides Broad Cell-Intrinsic Antiviral Immunity through Multihit Host-Directed Targeting of the Sterol Pathway, PLoS Biol. 2016 Mar 3;14(3):e1002364. doi: 10.1371/journal.pbio.1002364. eCollection 2016.

2015

William J. Griffiths, Jonas Abdel-Khalik, Peter J. Crick, Eylan Yutuc, Yuqin Wang, New methods for analysis of oxysterols and related compounds by LC–MS, The Journal of Steroid Biochemistry and Molecular Biology, Available online 27 November 2015, On print September 2016, Volume 162, Pages 4–26, http://dx.doi.org/10.1016/j.jsbmb.2015.11.017

Peter J. Crick, T. William Bentley, Yuqin Wang, William J. Griffiths, Revised sample preparation for the analysis of oxysterols by enzyme-assisted derivatisation for sterol analysis (EADSA), Analytical and Bioanalytical Chemistry, July 2015, Volume 407, Issue 17, pp 5235-5239
Keywords: Sample preparation Solid-phase extraction Lipidomics Cholesterol Oxysterol Derivatisation Liquid chromatography Mass spectrometry

Luigi Iuliano, Peter J. Crick, Chiara Zerbinati, Luigi Tritapepe, Jonas Abdel-Khalik, Marc Poirot, Yuqin Wang, William J. Griffiths, Cholesterol metabolites exported from human brain , Steroids, Volume 99, Part B, July 2015, Pages 189-193, ISSN 0039-128X, http://dx.doi.org/10.1016/j.steroids.2015.01.026.
Keywords: Oxysterol; LC–MS; GC–MS; 24S-hydroxycholesterol

Peter J. Crick, Lien Beckers, Myriam Baes, Paul P. Van Veldhoven, Yuqin Wang, William J. Griffiths, The oxysterol and cholestenoic acid profile of mouse cerebrospinal fluid, Steroids, Volume 99, Part B, July 2015, Pages 172-177, ISSN 0039-128X, http://dx.doi.org/10.1016/j.steroids.2015.02.021.
Keywords: LC–MS; Derivatisation; Bile acid; Brain

William J Griffiths, Jonas Abdel-Khalik, Peter J. Crick, Eylan Yutuc, Yuqin Wang. New methods for analysis of oxysterols and related compounds by LC-MS. The Journal of Steroid Biochemistry and Molecular Biology, Available online 27 November 2015, ISSN 0960-0760, http://dx.doi.org/10.1016/j.jsbmb.2015.11.017.

2014

Theofilopoulos S, Griffiths WJ, Crick PJ, Yang S, Meljon A, Ogundare M, Kitambi SS, Lockhart A, Tuschl K, Clayton PT, Morris AA, Martinez A, Reddy MA, Martinuzzi A, Bassi MT, Honda A, Mizuochi T, Kimura A, Nittono H, De Michele G, Carbone R, Criscuolo C, Yau JL, Seckl JR, Schüle R, Schöls L, Sailer AW, Kuhle J, Fraidakis MJ, Gustafsson JA, Steffensen KR, Björkhem I, Ernfors P, Sjövall J, Arenas E, Wang Y. Cholestenoic acids regulate motor neuron survival via liver X receptors. J Clin Invest. 2014 Oct 1. pii: 68506. doi: 10.1172/JCI68506. PubMed PMID: 25271621.

Saeed AA, Genove G, Li T, Luetjohann D, Olin M, Mast N, Pikuleva IA, Crick P, Wang Y, Griffiths W, Betsholtz C, Björkhem I. Effects of a disrupted blood-brain barrier on cholesterol homeostasis in the brain. J Biol Chem. 2014 Jun 27. pii: jbc.M114.556159. PubMed PMID: 24973215.

Wang Y, Karu K, Meljon A, Turton J, Yau JL, Seckl JR, Wang Y, Griffiths WJ. 24S,25-Epoxycholesterol in mouse and rat brain. Biochem Biophys Res Commun. 2014 Jun 27;449(2):229-34. doi: 10.1016/j.bbrc.2014.05.012. Epub 2014 May 14. PubMed PMID: 24832732; PubMed Central PMCID: PMC4053837.

Björkhem I, Diczfalusy U, Lövgren-Sandblom A, Starck L, Jonsson M, Tallman K, Schirmer H, Ousager LB, Crick PJ, Wang Y, Griffiths WJ, Guengerich FP. On the formation of 7-ketocholesterol from 7-dehydrocholesterol in patients with CTX and SLO. J Lipid Res. 2014 Apr 25;55(6):1165-1172.  PubMed PMID: 24771866; PubMed Central PMCID: PMC4031947.

Autio KJ, Schmitz W, Nair RR, Selkälä EM, Sormunen RT, Miinalainen IJ, Crick PJ, Wang Y, Griffiths WJ, Reddy JK, Baes M, Hiltunen JK. Role of AMACR (α-methylacyl-CoA racemase) and MFE-1 (peroxisomal multifunctional enzyme-1) in bile acid synthesis in mice. Biochem J. 2014 Jul 1;461(1):125-35. doi: 10.1042/BJ20130915. PubMed PMID: 24735479.

Rigdova K, Wang Y, Ward M, Griffiths WJ. A new derivative for oxosteroid analysis by mass spectrometry. Biochem Biophys Res Commun. 2014 Apr 11;446(3):762-7. doi: 10.1016/j.bbrc.2014.01.190. Epub 2014 Feb 10. PubMed PMID: 24525129; PubMed Central PMCID: PMC4000438.

Crick PJ, Aponte J, Bentley TW, Matthews I, Wang Y, Griffiths WJ. Evaluation of novel derivatisation reagents for the analysis of oxysterols. Biochem Biophys Res Commun. 2014 Apr 11;446(3):756-61. doi: 10.1016/j.bbrc.2014.01.173. Epub 2014 Feb 10. PubMed PMID: 24525124; PubMed Central PMCID: PMC4000439.

Meljon A, Wang Y, Griffiths WJ. Oxysterols in the brain of the cholesterol 24-hydroxylase knockout mouse. Biochem Biophys Res Commun. 2014 Apr 11;446(3):768-74. doi: 10.1016/j.bbrc.2014.01.153. Epub 2014 Jan 31. PubMed PMID: 24491562; PubMed Central PMCID: PMC4000437.

Abdel-Khalik J, Crick PJ, Carter GD, Makin HL, Wang Y, Griffiths WJ. Studies on the analysis of 25-hydroxyvitamin D(3) by isotope-dilution liquid chromatography-tandem mass spectrometry using enzyme-assisted derivatisation. Biochem Biophys Res Commun. 2014 Apr 11;446(3):745-50. doi: 10.1016/j.bbrc.2014.01.088. Epub 2014 Jan 30. PubMed PMID: 24486315; PubMed Central PMCID: PMC4000436.

2013

Blanc M, Hsieh WY, Robertson KA, Kropp KA, Forster T, Shui G, Lacaze P, Watterson S, Griffiths SJ, Spann NJ, Meljon A, Talbot S, Krishnan K, Covey DF, Wenk MR, Craigon M, Ruzsics Z, Haas J, Angulo A, Griffiths WJ, Glass CK, Wang Y, Ghazal P. The transcription factor STAT-1 couples macrophage synthesis of 25-hydroxycholesterol to the interferon antiviral response. Immunity. 2013 Jan 24;38(1):106-18. doi: 10.1016/j.immuni.2012.11.004. Epub 2012 Dec 27. PubMed PMID: 23273843; PubMed Central PMCID: PMC3556782.

Theofilopoulos S, Wang Y, Kitambi SS, Sacchetti P, Sousa KM, Bodin K, Kirk J, Saltó C, Gustafsson M, Toledo EM, Karu K, Gustafsson JÅ, Steffensen KR, Ernfors P, Sjövall J, Griffiths WJ, Arenas E. Brain endogenous liver X receptor ligands selectively promote midbrain neurogenesis. Nat Chem Biol. 2013 Feb;9(2):126-33. doi: 10.1038/nchembio.1156. Epub 2012 Dec 23. PubMed PMID: 23292650.

2012
Meljon A, Theofilopoulos S, Shackleton CH, Watson GL, Javitt NB, Knölker HJ, Saini R, Arenas E, Wang Y, Griffiths WJ. Analysis of bioactive oxysterols in newborn mouse brain by LC/MS. J Lipid Res. 2012 Nov;53(11):2469-83.

Griffiths WJ, Crick PJ, Wang Y, Ogundare M, Tuschl K, Morris AA, Bigger BW, Clayton PT, Wang Y. Analytical strategies for characterization of oxysterol lipidomes: Liver X receptor ligands in plasma. Free Radic Biol Med. 2012 Jul 27. [Epub ahead of print].

2011
Griffiths WJ, Wang Y. Analysis of oxysterol metabolomes. Biochim Biophys Acta. 2011 Nov;1811(11):784-99.

Karu K, Turton J, Wang Y, Griffiths WJ. Nano-liquid chromatography-tandem mass spectrometry analysis of oxysterols in brain: monitoring of cholesterol autoxidation. Chem Phys Lipids. 2011 Sep;164(6):411-24.

Griffiths WJ, Ogundare M, Williams CM, Wang Y. On the future of “omics”: lipidomics. J Inherit Metab Dis. 2011 Jun;34(3):583-92.

2010
Ogundare M, Theofilopoulos S, Lockhart A, Hall LJ, Arenas E, Sjovall J, Brenton AG, Wang Y, Griffiths WJ. Cerebrospinal fluid steroidomics: Are bioactive bile acids present in brain? J Biol Chem. 2010 Feb 12;285(7):4666-79.

2009
Wang Y, Sousa KM, Bodin K, Theofilopoulos S,Sacchetti P, Hornshaw M, Woffendin G, Karu K, Sjövall J, Arenas E and Griffiths WJ. 2009 Targeted lipidomic analysis of oxysterols in the embryonic central nervous system. Molecular BioSystems, 2009 May;5(5):529-41

Seibert C, Davidson BR, Fuller BJ, Patterson LH, Griffiths WJ, Wang Y. Multiple-Approaches to the Identification and Quantification of Cytochromes P450 in Human Liver Tissue by Mass Spectrometry. J Proteome Res. 2009 Apr;8(4):1672-81

Wang Y, Mulligan C, Denyer G, Delom F, gna-Bricarelli F, Tybulewicz VL, Fisher EM, Griffiths WJ, Nizetic D, and Groet J. Quantitative proteomic characterisation of a mouse embryonic stem cell model of Down syndrome. Mol. Cell Proteomics. 2009 Apr;8(4):585-95

2008
Griffiths WJ, Hornshaw M, Woffendin G, Baker SF, Lockhart A, Heidelberger S, Gustafsson M, Sjövall J, Wang Y. Discovering oxysterols in plasma: a window on the metabolome. J Proteome Res. 2008 Aug;7(8):3602-12.

Griffiths WJ, Wang Y, Karu K, Samuel E, McDonnell S, Hornshaw M, Shackleton C. Potential of sterol analysis by liquid chromatography-tandem mass spectrometry for the prenatal diagnosis of Smith-Lemli-Opitz syndrome. Clin Chem. 2008 Aug;54(8):1317-24.

Wang Y, Muneton S, Sjövall J, Jovanovic JN, Griffiths WJ. The effect of 24S-hydroxycholesterol on cholesterol homeostasis in neurons: quantitative changes to the cortical neuron proteome. J Proteome Res. 2008 Apr;7(4):1606-14.

2007
Lane CS, Wang Y, Betts R, Griffiths WJ, Patterson LH.  Mol Cell Proteomics. 2007 Jun;6(6):953-62.

Karu K, Hornshaw M, Woffendin G, Bodin K, Hamberg M, Alvelius G, Sjövall J, Turton J, Wang Y, Griffiths WJ. Liquid chromatography-mass spectrometry utilizing multi-stage fragmentation for the identification of oxysterols. J Lipid Res. 2007 Apr;48(4):976-87.